壳聚糖功能化纤维膜固定辣根过氧化物酶用于催化氧化对硝基苯酚研究

Abstract

A hydrophilic fibrous membrane consisting of a network of a non-crystalline hydrophobic polymer and an in situ crosslinked hydrophilic chitosan polymer is studied. This chitosan-functionalized membrane can be employed as a support for immobilization of horseradish peroxidases (HRPs). The immobilized HRPs (mass loading ~110 mg /g) show high stabilities over a wide pH range of 4~10 and temperature range of 25~60°C. In addition, the immobilized HRPs can function as a recyclable biocatalyst for catalytic oxidation of p-nitrophenol (PNP). After 10 cycles, the PNP removal efficiency is 70 percent. The results demonstrate that the chitosan-functionalized fibrous membrane is a promising support for immobilization of enzymes and has applications related to enzyme catalysis.