Identifying and validating of prognostic genes associated with myeloid cell differentiation in cervical cancer: development of a risk model based on single-cell RNA sequencing combined with bulk RNA sequencing data
Xinyi Luo, Yifan Zhang, Yiquan Lu, Nan Wang, Fengjie Hao, Yongjun Chen, Xiaochun Fei, Junqing Wang
Journal：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
IF：5.6
DOI：10.3390/ijms27114685
PMID：42278217
Published：2026-05-22
research field：癌症生物学分子肿瘤学肝脏病学信号转导细胞力学
Abstract
The online database was used to analyze the expression level of GOLPH3 transcriptome, and the expression level of GOLPH3 protein in PRAD tissues and cells was detected by western blotting. PC-3 and LNCaP cells were selected to construct the GOLPH3 overexpression PRAD cells. CCK8 assay, EdU staining, colony formation assay, and a nude mouse xenograft model were used to evaluate the proliferation of PRAD cells. Glucose metabolism activity was determined by lactate release, intracellular glucose level, oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
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