lncRNA linc00893在糖尿病视网膜病变中的临床意义和生物学作用

Abstract

To examine the diagnostic value and mechanism of action of serum linc00893 in patients with diabetic retinopathy (DR), 209 diabetic patients, including 106 patients with DR, were included in the study. ROC curves assessed the clinical value of linc00893 in predicting DR, and logistic analyzed the factors affecting DR. RT-qPCR detected serum linc00893 and miR-107 expression, CCK8 measured cell proliferation, flow cytometry documented apoptosis, and ELISA detected the inflammatory factor. Dual luciferase reporter assay and RIP assay verified the interactions between linc00893 and miR-107. Biochemical indicators are more severe in DR than in diabetes alone, and linc00893 expression was notably down-regulated in DR patients. linc00893 is an influential factor in the promotion of DR and has diagnostic value for it. Transfection of linc00893 enhanced the proliferative capacity of high-glucose (HG) cells, reduced apoptosis, and lowered the levels of inflammatory factors (VEGF, IL-1β, IL-18). The results also demonstrated that miR-107 is a target gene of linc00893 and the two can directly bind. miR-107 was markedly up-regulated in DR patients and had a clear negative correlation with linc00893 expression. miR-107 overexpression inhibited HG cell proliferation and increased apoptosis and inflammatory. linc00893 is lowly expressed in DR patients, and it has the diagnostic value of predicting DR. miR-107 is involved in the DR mechanism as linc00893, which is manifested by low-linc00893 promoting miR-107 induced slowing down of cell proliferation, increasing apoptosis and inflammation level, which triggers DR.