The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

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Flow cytometric analysis of Ezrin expression in HAP-1 cells using Ezrin antibody . Green, isotype control; red, Ezrin.

Western blotting analysis using ezrin antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ezrin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using ezrin antibody . Ezrin expression in wild-type (WT) and ezrin (EZR) knockout (KO) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ezrin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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