Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

推荐稀释比 WB:1/500-1/1000; ICC/IF:1/100; FC:1/200-1/500; IP:1/20-1/50

Western blot analysis of Cdk4 in 3T3, Hela lysates using CDK4 antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.