^{BAPTA是一种钙选择性螯合剂，对Ca2+的选择性比Mg2+高100倍以上。它基本的螯合单位和EDTA类似，只是两个脂肪氮被芳香氮所替代。因此，BAPTA在生理pH下不会被质子化。BAPTA的pKa3为5.47，pKa4为6.36。这个特点显示了去质子化的步骤并不包含在Ca的螯合步骤中。由于不受质子的干扰，它的螯合率比EGTA要高很多。BAPTA-AM是BAPTA的乙酰甲酯衍生物，通过AM法，能被轻易负载到细胞中。BAPTA-AM对于细胞内钙离子的监控是非常有用的。}

参考文献

^{[1] Lei M, Wang W, Zhang H, Gong J, Cai H, Wang Z, Zhu L, Yang X, Wang S, Ma C. Piezo1 Regulates Stiffness-Dependent DRG Axon Regeneration via Modifying Cytoskeletal Dynamics. Adv Sci (Weinh). 2024 Dec;11(47):e2405705. doi: 10.1002/advs.202405705. Epub 2024 Nov 8. PMID: 39514408; PMCID: PMC11653623. (IF:14.98)}

^{[2]1Wie MB, et al. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures.Prog Neuropsychopharmacol Biol Psychiatry. 2001,25(8):1641-1659.                          
[3]1Tang Q, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells.Biochem Pharmacol. 2007, 74(11), 1596-1607.                            
[4]1Marzioni M, et al. Ca2+-dependent cytoprotective effects of ursodeoxycholic and tauroursodeoxycholic acid on the biliary epithelium in a rat model of cholestasis and loss of bile ducts.Am J Pathol. 2006, 168(2), 398-409.}

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^{[1] Lei M, Wang W, Zhang H, Gong J, Cai H, Wang Z, Zhu L, Yang X, Wang S, Ma C. Piezo1 Regulates Stiffness-Dependent DRG Axon Regeneration via Modifying Cytoskeletal Dynamics. Adv Sci (Weinh). 2024 Dec;11(47):e2405705. doi: 10.1002/advs.202405705. Epub 2024 Nov 8. PMID: 39514408; PMCID: PMC11653623. (IF:14.98)}

^{[2]1Wie MB, et al. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures.Prog Neuropsychopharmacol Biol Psychiatry. 2001,25(8):1641-1659.                          
[3]1Tang Q, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells.Biochem Pharmacol. 2007, 74(11), 1596-1607.                            
[4]1Marzioni M, et al. Ca2+-dependent cytoprotective effects of ursodeoxycholic and tauroursodeoxycholic acid on the biliary epithelium in a rat model of cholestasis and loss of bile ducts.Am J Pathol. 2006, 168(2), 398-409.}