cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Immunocytochemical staining of HAP-1 cells with PRKAR2A antibody . Nuclei were stained blue with DAPI;PRKAR2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using PRKAR2A antibody . Green, isotype control; red, PRKAR2A.

Western blotting analysis using PRKAR2A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKAR2A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using PRKAR2A antibody . PRKAR2A expression in wild-type (WT) and PRKAR2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRKAR2A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of PRKAR2A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PRKAR2A antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using PRKAR2A antibody , Top panel: wild-type (WT); Bottom panal: PRKAR2A shRNA knockdown (KD). Nuclei were stained blue with DAPI; PRKAR2A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Store at -20℃ for one year.