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Flow cytometric analysis of DDB2 expression in HT-1080 cells using DDB2 antibody . Green, isotype control; red, DDB2.
Validation of DDB2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDB2 antibody and analyzed using BD flow cytometer.
Immunocytochemical staining of HT-1080 cells with DDB2 antibody . Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using DDB2 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using DDB2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . DDB2, damage specific DNA binding protein 2.
Western blotting analysis using DDB2 antibody . DDB2 expression in wild type (WT) and DDB2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
