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Flow cytometric analysis of LPCAT1 expression in HepG2 cells using LPCAT1 antibody . Green, isotype control; red, LPCAT1.
Immunocytochemical staining of HepG2 cells with LPCAT1 antibody . Nuclei were stained blue with DAPI; LPCAT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using LPCAT1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LPCAT1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using LPCAT1 antibody . LPCAT1 expression in wild-type (WT) and LPCAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LPCAT1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Validation of LPCAT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with LPCAT1 antibody and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using LPCAT1 antibody , Top panel: wild-type (WT); Bottom panal: LPCAT1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LPCAT1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
