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Immunocytochemical staining of C2C12 cells with Zinc fingers and homeoboxes 2 antibody . Nuclei were stained blue with DAPI; Zinc fingers and homeoboxes 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Zinc fingers and homeoboxes 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Zinc fingers and homeoboxes 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using Zinc fingers and homeoboxes 2 antibody . Zinc fingers and homeoboxes 2 expression in wild type (WT) and Zinc fingers and homeoboxes 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Zinc fingers and homeoboxes 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Validation of Zinc fingers and homeoboxes 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Zinc fingers and homeoboxes 2 antibody and analyzed using BD flow cytometer.
