本产品仅用作科学研究！
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using phosphoglycerate dehydrogenase antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HeLa cells using Phosphoglycerate dehydrogenase antibody , Top panel: wild-type (WT); Bottom panal: Phosphoglycerate dehydrogenase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phosphoglycerate dehydrogenase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using phosphoglycerate dehydrogenase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphoglycerate dehydrogenase antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using phosphoglycerate dehydrogenase antibody . Phosphoglycerate dehydrogenase expression in wild-type (WT) and phosphoglycerate dehydrogenase (PHGDH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phosphoglycerate dehydrogenase antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Validation of Phosphoglycerate dehydrogenase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phosphoglycerate dehydrogenase antibody and analyzed using BD flow cytometer.
