Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel, p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

推荐稀释比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/100-1/200

Western blot analysis of Phospho-NF-KB p65 (Thr254) in 3T3 lysates untreated or treated with TNFα using NFκBp65 (Phospho-Thr254) antibody.

Immunofluorescence analysis of Phospho-NF-KB p65 (Thr254) in Hela using NFκBp65(Phospho-Thr254) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using NFκBp65 (Phospho-Thr254) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Phospho-NF-KB p65 (Thr254) in 3T3 lysates untreated or treated with Alkaline phosphatase using Phospho-NF-KB p65 (Thr254) antibody(upper)or βActin antibody(2000688F10, lower).

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