Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

推荐稀释比 WB:1/1000-1/2000; IHC:1/50-1/100

Western blot analysis of Phospho-MLKL (S358) in (1) HT-29 lysates; (2) HT-29 lysates treated with TNF alpha+ Smac mimetic+ zVAD using Phospho-MLKL (Ser358) antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.