T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by uses T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA.
This kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.
l High yield: can produce up to 200 μg in 2 hours through transcription reaction
l Better versatility: suitable for transcription of 20nt-10000nt RNA
l Excellent performance: effectively reduce the byproducts of transcription during IVT process
l Multiple applications: can be used for ordinary, labeled, and modified RNA synthesis
1、
Figure 1: Yield of transcripts of different lengths
Figure 2: Quality of transcripts of different lengths
Figure 4: Expression of transcribed mRNA in HEK-293 cells
Note:The mRNA has been capped with Vaccinia Capping Enzyme(Yeasen#10614/10615).
Store at -20℃, valid for two years.
[1] Dong Z, Zheng N, Hu C, et al. Nosema bombycis microRNA-like RNA 8 (Nb-milR8) Increases Fungal Pathogenicity by Modulating BmPEX16 Gene Expression in Its Host, Bombyx mori. Microbiol Spectr. 2021;9(2):e0104821. doi:10.1128/Spectrum.01048-21(IF:7.171)
[2] Wang X, Tang S, Ye S, et al. Ultrasensitive quantitation of circulating miR-195-5p with triple strand displacement amplification cascade. Talanta. 2022;242:123300. doi:10.1016/j.talanta.2022.123300(IF:6.057)
