分子生物学
IVD分子诊断
细胞培养与分析
蛋白研究
细胞因子
重组蛋白
抗体
高通量测序建库
病原检测UCF系列
生物医药
工具酶
抑制剂激活剂与常用试剂
仪器
耗材

VvTOR Responds to ABA Signal and Affects Sugar Related Genes Expression in Grape

Zhao Y., Wang X.-Q.

Journal:RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

IF:1.42

DOI:10.1134/S1021443722020236

PMID:

Published:2022-03-18

research field:基因组编辑分子生物学精准医学CRISPR-Cas系统基因治疗肝脏疾病心血管疾病

Abstract

Abstract The target of rapamycin (TOR) signalling network plays a pivotal role in sugar metabolism and plants growth. In this article, we made use of the grape ( Vitis vinifera ) calli as experiment materials to explore the function of VvTOR gene and protein. By dual-luciferase reporter system, We found that VvTOR promoter could respond to ABA (Abscisic Acid) signal and VvTOR 1200 bp promoter fragment had stronger activity. By the treatment of AZD8055, an inhibitor of VvTOR, the sugars (glucose, sucrose, fructose) contents of grape calli had different changes and the growth of grape calli could be repressed. We further found that VvTOR could affect grape growth and sugar metabolism genes expression under the different treatments of ABA and AZD8055. These results provide a new evidence to support the viewpoint that VvTOR could respond to ABA signal and affect sugar related genes expression in grapes.

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