翌圣生物 Yeasen｜生命科学试剂与诊断原料一站式平台

Abstract

The widespread global distribution of saline-alkali land poses a major challenge to agricultural production and water resource management. Saline-alkali stress is extremely harmful to the growth of most plants, but green algae can survive in saline-alkali soil. A saline-alkali tolerant (SAT) Chlorella strain that shows strong sodium bicarbonate (NaHCO 3 ) tolerance was isolated previously. In this study, we cloned the gene ( ChaccD ) encoding the carboxyltransferase β subunit of acetyl-CoA carboxylase from SAT Chlorella by screening a full-length yeast cDNA library constructed from cells exposed to NaHCO 3 . The ChaccD expression level was elevated in response to NaHCO 3 treatment and the ChaccD protein was localized to the chloroplasts. ChaccD -transgenic rice and ChaccD -overexpressing Chlorella showed stronger NaHCO 3 tolerance, and improved crude fat and free fatty acid contents were observed in mature rice grains and Chlorella cells treated with NaHCO 3 . These data indicated that ChaccD is a candidate to confer saline-alkali tolerance for cultivation and nutrition of rice in saline-alkali soil. In addition, ChaccD may be involved in the conversion of NaHCO 3 into lipids, which could be relevant to the production of biodiesel feedstocks, though further validation is required.