Abstract

Angelica sinensis polysaccharide (ASP) is one of the principal active components of Angelica sinensis (AS) that is widely used in natural medicine and has various pharmacological activities, including antioxidant, anti-inflammatory, and enhancing immunity. However, its pharmacological role of anti-aging needs to be clarified. Here, we detected the beneficial effect and mechanism of ASP on healthy aging and aging-related diseases using the Drosophila melanogaster model. The results showed that oral administration of ASP remarkably extended lifespan, increased reproduction, improved climbing ability, and increased resistance to starvation and oxidative stress in aged flies, mainly via inhibiting insulin signaling (IIS) and TOR signaling and boosting antioxidant ability. Further, ASP supplementation protected against aging-induced intestinal homeostasis imbalance via inhibiting intestinal stem cells (ISCs) hyperproliferation and oxidative damage, improved sleep disorders via rescuing sleep rhythm in aged flies, and had a neuroprotective effect on Aβ42 transgenic flies. Taken together, our findings shed light on the possibility that ASP could increase lifespan, improve healthy aging, and ultimately reduce the incidence of age-related illnesses. It holds promise as a candidate for anti-aging intervention and treatment for aging-associated disorders.