马尾松CCCH型锌指蛋白家族全转录组鉴定及RR-TZF蛋白在胁迫应答中的功能研究

Abstract

CCCH-type zinc finger proteins play an important role in multiple biotic and abiotic stresses. More and more reports about CCCH functions in plant development and stress responses have appeared over the past few years, focusing especially on tandem CCCH zinc finger proteins (TZFs). However, this has not been reported in Pinaceae. In this study, we identified 46 CCCH proteins, including 6 plant TZF members inPinus massoniana, and performed bioinformatic analysis. According to RT-PCR analysis, we revealed the expression patterns of five RR-TZF genes under different abiotic stresses and hormone treatments. Meanwhile, tissue-specific expression analysis suggested that all genes were mainly expressed in needles. Additionally, RR-TZF genes showed transcriptional activation activity in yeast. The results in this study will be beneficial in improving the stress resistance ofP. massonianaand facilitating further studies on the biological and molecular functions of CCCH zinc finger proteins.Keywords:CCCH zinc finger proteins;tandem CCCH zinc finger;P. massoniana;abiotic stresses;expression patterns