过氧化物酶体定位的GmABCA7过表达促进拟南芥种子萌发

Abstract

Peroxisome is one of the important organelles for intracellular lipid metabolism in plant cells and β-oxidation of fatty acids in peroxisomes provides the energy for oil-containing seed germination. In this study, we identified an ATP-binding cassette (ABC) transporter gene,GmABCA7from soybean, which is highly expressed in the different developmental stages of seeds. Transient expression ofGmABCA7in tobacco epidermal cells showed that GmABCA7 was specifically localized at the peroxisomes. Overexpression ofGmABCA7in Arabidopsis does not change seed phenotypes, or the overall levels of lipid, protein and sugar stored in the seeds; however, the transgenic seeds produced more gluconeogenic pathway precursors such as succinate and malate and germinated earlier compared to the wild type seeds. These results suggest that GmABCA7 may affect the β-oxidation of fatty acids and play an important role in seed germination.Keywords:peroxisome;β-oxidation;seed germination;GmABCA7;soybean