利用酵母表面展示技术开发加州鲈鱼口服疫苗抗LMBV感染

Abstract

Simple SummaryLMBV is an important pathogen in the breeding process of largemouth bass, but there is no effective control method for it. This study developed oral vaccines for LMBV. The vaccines can significantly increase the activity of immune-related enzymes, upregulate the expression of immune genes, and stimulate the production of neutralizing antibodies in serum in largemouth bass. In addition, the vaccines can reduce the mortality of LMBV infection. These results indicate that the vaccines are expected to be candidate vaccines for controlling LMBV infection.AbstractLargemouth bass ranavirus (LMBV) infects largemouth bass, leading to significant mortality and economic losses. There are no safe and effective drugs against this disease. Oral vaccines that directly target the intestinal mucosal immune system play an important role in resisting pathogens. Herein, the B subunit ofEscherichia coliheat-labile enterotoxin (LTB, a mucosal immune adjuvant) and the LMBV main capsid protein (MCP) were expressed usingSaccharomyces cerevisiaesurface display technology. The yeast-prepared oral vaccines were named EBY100-OMCP and EBY100-LTB-OMCP. The candidate vaccines could resist the acidic intestinal environment. After 7 days of continuous oral immunization, indicators of innate and adaptive immunity were measured on days 1, 7, 14, 21, 28, 35, and 42. High activities of immune enzymes (T-SOD, AKP, ACP, and LZM) in serum and intestinal mucus were detected.IgMin the head kidney was significantly upregulated (EBY100-OMCP group: 3.8-fold; BY100-LTB-OMCP group: 4.3-fold).IgTwas upregulated in the intestines (EBY100-OMCP group: 5.6-fold; EBY100-LTB-OMCP group: 6.7-fold). Serum neutralizing antibody titers of the two groups reached 1:85. Oral vaccination protected against LMBV infection. The relative percent survival was 52.1% (EBY100-OMCP) and 66.7% (EBY100-LTB-OMCP). Thus, EBY100-OMCP and EBY100-LTB-OMCP are promising and effective candidate vaccines against LMBV infection.Keyw