Discovery and characterization of a novel splice variant of the p53 tumor suppressor gene in a human T cell leukemia cellline
Xiaomei Li, Yingshou Lei, Yang Yu, Yaqian Zhang, Wenfeng Zhang, Han Shen, Changli Tao, Fenglin Wu, Shulin Huang, Hongwei Shao
Journal：International Journal of Clinical and Experimental Pathology
IF：0.25
DOI：PMID:32509087
PMID：32509087
Published：2020-05-01
research field：肿瘤学药学纳米技术材料科学
Abstract
Alternative splicing produces multiple mRNA variants of TP53 which have diverse biologic functions. In this study, we identified a novel splice variant of TP53 lacking a 200 nt portion of exon 4 (p53ΔE4p) from a human leukemia T cell line. No protein product of p53ΔE4p was identifiable by western blot; however, forced expression of the variant in HEK-293T cells expressing wild-type p53 could inhibit cell proliferation and promote cell death. Interestingly, this novel variant also significantly enhances the expression of reporter genes. Moreover, transcriptome analysis showed that genes related to DNA binding and regulation of transcription by RNA polymerase II function were significantly upregulated following p53ΔE4p transfection, suggesting a role for this variant in the regulation of gene expression.
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