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RAFTER: a releasing factor tethered RNA editing system for quantifying mRNA translation
Zhiyuan Sun, Peng Dong, Huanyu Yan, Xiaozhen Wen, Yanping Li, Feifei Jiang, Liang Fang, Xi Wang, Wei Chen
Journal：Life Science Alliance
IF：3.1
DOI：10.26508/lsa.202603651
PMID：42493246
Published：2026-07-23
research field：可持续农业微生物学微生物生态学分子植物-微生物互作植物病理学
Abstract
Measurement of mRNA translation level has heavily relied on quantifying the amount of polysome-associated RNAs, which would not always reflect efficient translation. To address this, we developed ReleAsing Factor Tethered Editing of RNA (RAFTER), which combined the RNA editing enzyme with translation releasing factor to selectively label successfully translated RNAs. The translation level estimated based on RAFTER correlated well with that by Ribo-seq and Polysome-seq, but with a greatly simplified workflow, which enables it for single-cell analysis.
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