CRISPR-CasRx knock-in mice for RNA degradation
Li Jiacheng, Zhu Dekun, Hu Shengshou, Nie Yu
Journal：Science China-Life Sciences
IF：10.37
DOI：10.1007/s11427-021-2059-5
PMID：35412223
Published：2022-04-07
research field：分子生物学免疫学传染病学微生物学
Abstract
The RNA editing tool CRISPR-CasRx has provided a platform for a range of transcriptome analysis tools and therapeutic approaches with its broad efficacy and high specificity. To enable the application of CasRx in vivo , we established a Credependent CasRx knock-in mouse. Using these mice, we specifically knocked down the expression of Meis1 and Hoxb13 in cardiomyocytes, which induced cardiac regeneration after myocardial infarction. We also knocked down the lncRNA Mhrt in cardiomyocytes with the CasRx knock-in mice, causing hypertrophic cardiomyopathy. In summary, we generated a Credependent CasRx knock-in mouse that can efficiently knock down coding gene and lncRNA expression in specific somatic cells. This in vivo CRISPR-CasRx system is promising for gene function research and disease modeling.
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