Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types.

Immunocytochemistry

Immunocytochemistry analysis of STAT5b (green) in Hela using STAT5b antibody,and DAPI(blue).

Western

Western blot analysis of STAT5b in Raw264.7, PC-12 lysates using STAT5b antibody.

Western

Western blot analysis of STAT5b in Hela, A549, HL-60 lysates using STAT5b antibody

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using STAT5b antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.