Recombinant Human OX40 Ligand/TNFSF4 Protein,hFc Tag

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产品说明书

FAQ

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已发表文献

性能参数

 

蛋白别名(Synonyms)

OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;?TXGP1; Glycoprotein Gp34

种属(Species)

Human

表达系统(Source)

HEK293

表达序列(Sequence)

Gln51-Leu183

Accession

P23510-1

标签(Tag)

N-hFc

分子量(Molecular Weight)

The protein has a predicted MW of 40.95 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC

活性(Activity)

ELISA Data: Immobilized Biotinylated Human OX40, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA .

SPR Data: Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay .

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外观(Appearance)

冻干粉

 

储存条件

 

-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。

 

复溶方法

 

开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。

 

注意事项

 

  1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
  2. 本产品仅作科研用途。

 

 

Ver. CN20240905

 

 

 

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