Recombinant Human Activin RIIA/ACVR2AProtein,hFc Tag

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产品说明书

FAQ

COA

已发表文献

性能参数

 

蛋白别名(Synonyms)

ACTR-IIA; ACTRIIA; ACVR2A; ACVR2

种属(Species)

Human

表达系统(Source)

HEK293

表达序列(Sequence)

Ala20-Pro135

Accession

P27037-1

标签(Tag)

C-hFc

分子量(Molecular Weight)

The protein has a predicted MW of W 39.35 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC

活性(Activity)

ELISA Data: Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA . ELISA Data: Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 μg/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外观(Appearance)

冻干粉

 

储存条件

 

-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。

 

复溶方法

 

开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。

 

注意事项

 

  1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
  2. 本产品仅作科研用途。

 

 

 

Ver. CN20240905

 

 

 

 

 

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