Purity:SDS-PAGE

Anti-CLDN6 Reference Antibody (IMAB027) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

Purity:SEC-HPLC

The purity of Anti-CLDN6 Reference Antibody (IMAB027) is 98.41%, determined by SEC-HPLC.

Bioactivity:ELISA

Immobilized human CLDN6 VLP at 8 ug/mL can bind Anti-CLDN6 Reference Antibody (IMAB027), EC50=0.001599 ug/mL.

Bioactivity:FACS

Human CLDN6 CHO cells were stained with Anti-CLDN6 Reference Antibody (IMAB027) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=4.342 nM.

Function:ADCC

Anti-CLDN6 Reference Antibody (IMAB027) induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell. The max induction fold was approximately 14.16 .

Function:Internalization

Anti-CLDN6 Reference Antibody (IMAB027) by huCLDN6-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 1 nM.

Research in vivo

IMAB027 inhibited the tumor growth of OV90 on Balb/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 50.7% at 5 mpk at D34.

-25~-15℃保存，有效期1年。