噬菌体DNA中新型二胺丙烷修饰胸腺嘧啶的发现及生物合成机制

Abstract

Bacteriophage genomes exhibit exceptional diversity in nucleotide modifications, which primarily function to counteract host defense systems. However, the diversity of phage DNA hypermodifications remains largely unexplored in post genome era. Here, we discovered a novel thymine hypermodification, α-1,3-diaminopropanylthymine (α-dapT), in the Acinetobacter baumannii phage SH-Ab 15599, and elucidated its biosynthetic pathway, featuring the phage-encoded key diamine DNA transferase (DADT, formerly αGPT-Pplase2). DADT utilizes the metabolite 1,3-diaminopropane, which is the major polyamine in the host to modify phage DNA. DADT exhibits broad in vitro substrate specificity but a strong in vivo preference for 1,3-diaminopropane. Structural and mutagenesis analyses revealed the molecular basis for substrate recognition and catalysis. The α-dapT modification occurs preferentially at TG dinucleotides and confers resistance to multiple host restriction enzymes. Furthermore, RNA-seq analysis showed that phage infection upregulates genes for 1,3-diaminopropane synthesis, and downregulates genes for 1,3-diaminoproprane consumption to supply the modification precursor. Given that 1,3-diaminopropane functions as a key regulator in mobility of A. baumannii , its metabolic reprograming may impair host biofilm formation.