Abstract

Intestinal epithelial barrier function is vital to gut homeostasis. This study investigated the effects of Lacticaseibacillus rhamnosus 1.0320 combined with 3-FL and 3'-SL using the LPS-induced barrier injury model. The results showed that the combination of 3-FL, 3'-SL, and L. rhamnosus 1.0320 significantly enhanced the levels of GSH, SOD and CAT, reduced the levels of pro-inflammatory cytokine. Meanwhile, the combination ameliorated intestinal epithelial barrier dysfunction by upregulating expression levels of tight junction (TJ) protein. The underlying mechanism was that the combination inhibited the TLR4/NF-κB pathway by reducing MyD88 expression and NF-κB p65 phosphorylation. Meanwhile, the combination activated the Nrf2/NQO-1 signaling pathway by promoting Nrf2 nuclear translocation. Additionally, L. rhamnosus 1.0320 combined with 3-FL and 3'-SL reduced apoptosis by modulating the expression ratio of Bcl-2/Bax. These findings would provide a combined strategy for the development of functional foods to ameliorate intestinal barrier dysfunction.