Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis.

WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200  IP: 1/20

Immunocytochemistry

Immunocytochemistry analysis of Rab5 (green) in HT-1080 using Rab5 antibody,and DAPI(blue).

Western blot

Western blot analysis of Rab5 in K562, C6, 3T3, Hela lysates using Rab5 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Rab5 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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