This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100  ICC: 1/100-1/200  FC: 1/50-1/100

Western blot

Western blot analysis of hnRNP F in 293T(WT) and HNRNPF-Knockout 293T(KO) using hnRNP F antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human Kidney tissue using HNRNPF antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of hnRNP F in HepG2 using HNRNPF antibody.

Flow

Flow Cytometry analysis of Jurkat stained with HNRNPF antibody (Red).Blue line histogram represents nonspecific negative control.

-15到-25度