Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

推荐稀释比 WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Western

Western blot analysis of TriMethyl-Histone H3 (Lys4) in various cell lines lysates using TriMethyl-Histone H3-K4 antibody.

TriMethyl-Histone

TriMethyl-Histone H3 (Lys4) D198 Dotblot analysis of all sorts of methylation peptides using TriMethyl-Histone H3 (Lys4) antibody

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human rectal cancer using TriMethyl-Histone H3-K4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of TriMethyl-Histone H3 (Lys4) in 293T using TriMethyl-Histone H3-K4 antibody,and DAPI (blue).

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