性能参数
蛋白别名(Synonyms) |
INHBA; Activin A; |
种属(Species) |
Human |
表达系统(Source) |
HEK293 |
表达序列(Sequence) |
Ser21-Ser426 |
Accession |
P08476 |
标签(Tag) |
N-His |
分子量(Molecular Weight) |
The protein has a predicted MW of 13.0 kDa (mature form) and 32.0 kDa (pro form). Due to glycosylation and interchain disulfide bond, the protein migrates to 15 kDa (mature form) and 40-50 kDa (pro form) under reduced (R) condition based on Bis-Tris PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Bis-Tris PAGE |
活性(Activity) |
Cell Based Assay: Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50?for this effect is 30-40 ng/mL. |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
Ver. CN20240905