利用iCRISPR/Cas9系统建立ELTD1基因敲除人胚胎干细胞系FAHZUe001-A
Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
Qian Luo, Cong Wei, Yan Long, Meng Zhang, Wei Shan, Honghu Li, Shuyang Cai, Yulin Xu, Pengxu Qian, He Huang
Journal：Stem Cell Research
IF：2.02
DOI：10.1016/j.scr.2021.102350
PMID：34087984
Published：2021-04-15
research field：分子生物学真菌学进化生物学微生物学遗传学与基因组学植物病理学
Abstract
Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro .
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