工程化CatI内含子作为广适性顺式元件在作物育种和植物研究中的应用
Engineering the CatI intron creates a broadly applicable cis-element for crop breeding and plant research
Keli Qiu, Rui Xu, Jiankai Zhou, Jun Liu, Liang Shao, Biao Xie, Yansheng Tang, Xiaojian Peng, Pengcheng Wei, Haiyang Jiang, Yunfei Li, Pengfei Jiang
Journal：Crop Journal
IF：6.7
DOI：10.1016/j.cj.2026.05.003
PMID：
Published：2026-05-29
research field：分子生物学遗传学植物生物技术作物科学
Abstract
Some introns can act as cis -elements to enhance gene expression without altering promoter specificity. Here, we engineered the castor bean catalase 1 first intron ( CatI ) by mutagenesis and identified CatI-mu7 , which exhibited enhanced fusion-gene expression and improved splicing accuracy in Nicotiana benthamiana, Arabidopsis , Solanum lycopersicum , and rice. Knock-in of CatI-mu7 into HSP101 increased male fertility under heat stress in rice and Arabidopsis compared with wild-type and CatI knock-in controls, highlighting its potential applications in crop breeding.
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