This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of Cytochrome b5 reductase 3 expression in H9c2 cells using Cytochrome b5 reductase 3 antibody . Green, isotype control; red, Cytochrome b5 reductase 3.

Immunocytochemical staining of H9c2 cells with Cytochrome b5 reductase 3 antibody . Nuclei were stained blue with DAPI; Cytochrome b5 reductase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human glioblastoma using cytochrome b5 reductase 3 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using cytochrome b5 reductase 3 antibody . Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cytochrome b5 reductase 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using cytochrome b5 reductase 3 antibody . Cytochrome b5 reductase 3 expression in wild type (WT) and cytochrome b5 reductase 3 (CYB5R3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cytochrome b5 reductase 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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