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Flow cytometric analysis of vimentin expression in C2C12 cells using vimentin antibody . Green, isotype control; red, vimentin.
Immunocytochemical staining of C2C12 cells with Vimentin antibody . Nuclei were stained blue with DAPI; Vimentin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using vimentin antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vimentin antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using vimentin antibody . Vimentin expression in wild type (WT) and vimentin (VIM) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with vimentin antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using vimentin antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
