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Flow cytometric analysis of Heat shock transcription factor 1 expression in HepG2 cells using Heat shock transcription factor 1 antibody . Green, isotype control; red, Heat shock transcription factor 1.
Immunocytochemical staining of HepG2 cells with Heat shock transcription factor 1 antibody . Nuclei were stained blue with DAPI; Heat shock transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Heat shock transcription factor 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Heat shock transcription factor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using Heat shock transcription factor 1 antibody . Heat shock transcription factor 1 expression in wild type (WT) and heat shock transcription factor 1 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Heat shock transcription factor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Validation of Heat shock transcription factor 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with Heat shock transcription factor 1 antibody and analyzed using BD flow cytometer.
Immunocytochemical staining of 293T cells using Heat shock transcription factor 1 antibody , Top panel: wild-type (WT); Bottom panal: Heat shock transcription factor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Heat shock transcription factor 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
