CD3ε（CD3E）是T细胞表面的I型跨膜糖蛋白，含有一个免疫球蛋白样结构域和一个ITAM结构域。它与CD3γ、CD3δ、CD3ζ及T细胞受体共同组成TCR-CD3复合物，在抗原识别信号转导中起关键作用。编码ε、γ、δ的基因位于11号染色体同一基因簇中。CD3E对T细胞发育至关重要，其缺陷可导致严重免疫缺陷，并与女性1型糖尿病易感性相关。

在体外实验中，为实现T细胞的充分激活、增殖和分化，通常将OKT3与抗CD28抗体联用，分别提供第一信号和第二信号，拟体内T细胞激活的生理条件。

内毒素＜1 EU/mg

For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

Anti- Human CD3E,（OKT3）In Vivo Mab on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti- Human CD3E,（OKT3）In Vivo Mab is 99%,determined by SEC-HPLC.

Human CD3E-expressing Jurkat cells were stained with Anti- Human CD3E,（OKT3）In Vivo Mab, with isotype and secondary-only controls. Flow analysis showed specific binding of the antibody, with an EC50 of 0.353 nM.

Human CD3E-expressing Jurkat cells were stained with Anti- Human CD3E,（OKT3）In Vivo Mab. Compared to the isotype control and secondary-only control, a CD3E-positive population is apparent.

Upon receipt, store immediately at -20℃ or lower for 2 years. Store aliquots at -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.