This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene.

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Immunocytochemical staining of HepG2 cells with Casein kinase 2 beta antibody . Nuclei were stained blue with DAPI; Casein kinase 2 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Casein kinase 2 beta antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Casein kinase 2 beta antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Casein kinase 2 beta antibody . Casein kinase 2 beta expression in wild type (WT) and Casein kinase 2 beta shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Casein kinase 2 beta antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Casein kinase 2 beta antibody , Top panel: wild-type (WT); Bottom panal: Casein kinase 2 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 2 beta was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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