Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of Phospho-Histone H3.3 (S31) expression in HepG2 cells using Phospho-Histone H3.3 (S31) antibody . Green, isotype control; red, Phospho-Histone H3.3 (S31).

Immunocytochemical staining of HepG2 cells with Phospho-Histone H3.3 (S31) antibody . Nuclei were stained blue with DAPI; Phospho-Histone H3.3 (S31) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospho-Histone H3.3 (S31) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-Histone H3.3 (S31) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using phospho-histone H3.3 (S31) antibody . Phospho-histone H3.3 (S31) expression in wild type (WT) and H3-3A knockdown (KD) HSHC cells with 5 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-histone H3.3 (S31) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using phospho-histone H3.3 (S31) antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

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