This gene encodes the A subunit of lactate dehydrogenase enzyme which catalyzes the reversible conversion of pyruvate to lactate with the concomitant oxidation of NADH to NAD in anaerobic glycolysis. The protein is found predominantly in skeletal muscle and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. The human genome contains several non-transcribed pseudogenes of this gene.

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Flow cytometric analysis of Lactate dehydrogenase A expression in HepG2 cells using Lactate dehydrogenase A antibody . Green, isotype control; red, Lactate dehydrogenase A.

Western blotting analysis using lactate dehydrogenase A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lactate dehydrogenase A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using lactate dehydrogenase A antibody . Lactate dehydrogenase A expression in wild type (WT) and lactate dehydrogenase A (LDHA) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lactate dehydrogenase A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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