E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.

推荐稀释比 WB:1/500-1/1000;IF:1/50-1/200;IP:1/20

Immunocytochemistry analysis of Ubiquitin Protein Ligase E3A (green) in Hela using Ubiquitin Protein Ligase E3A antibody,and DAPI(blue).

Western blot analysis of UBE3A in mouse brain, rat brain lysates using UBE3A antibody.

Western blot analysis of UBE3A in A549, HL-60, U2OS lysates using UBE3A antibody

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.