Generation of a FTO gene knockout human embryonic stem cell line using CRISPR/Cas9 editing
Cong Wei, Qian Luo, Binsheng Wang, Yan Long, Meng Zhang, Wei Shan, Xiaohong Yu, Yulin Xu, Pengxu Qian, He Huang
Journal：Stem Cell Research
IF：2.02
DOI：10.1016/j.scr.2021.102362
PMID：34087991
Published：2021-04-22
research field：肿瘤学分子生物学药理学细胞生物学
Abstract
Fat mass and obesity-associated protein (FTO) is the first protein found to have the activity of N6-methyladenosine (m6A) demethylation. It has been reported that FTO was involved in different physiological and pathological processes, including stem cell differentiation, sex determination, tumorigenesis, and progression. To further understand the exact role of FTO in these processes, we generated a FTO knockout human embryonic stem cell (hESC) line by CRISPR/Cas9 mediated gene editing method. This cell line maintained normal karyotype, pluripotency, and trilineage differentiation potential, which are considered as a model for function studies of the FTO protein in hESC self-renewal and differentiation.
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