香蒲花粉果胶多糖PTPS-2-2的结构表征及其抑制肝细胞脂质积累活性研究

Abstract

The pollen of Typha angustifolia L. decoction was clinically used to treat hyperlipidemia in China. A pectin polysaccharide (PTPS-2-2) was obtained from T . angustifolia pollen through water extraction, ion-exchange chromatography, and gel chromatography. Structural characterization showed that PTPS-2-2 had a molecular weight of 54 kDa and was composed of rhamnose , arabinose , xylose , galactose , and galacturonic acid with a molar ratio of 11.5: 36.5: 4.1: 36.7: 11.2. PTPS-2-2 consisted of rhamnogalacturonan I (RG-I) and arabinogalactan II (AG-II) domains. Its backbone was predominantly composed of →4-α-D-Gal p A-(1 → 2)-α-L-Rha p -(1→, with branches of 1,3-Gal p , 1,6-Gal p , 1,3,6-Gal p , T-Ara f , 1.5-Ara f and T-Xyl p , connected to the 4-position of 1,2-Rha p and the 3-position of 1,4-Gal p A. The inhibitory effect of PTPS-2-2 on lipid accumulation was studied in vitro, using L02 cells induced by oleic acid . This experiment shows that PTPS-2-2 treatment at 100–400 μg/mL dose-dependently reduce cellular triglycerides (TG), cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA) levels, while elevated superoxide dismutase (SOD) levels. This indicated that PTPS-2-2 potentially ameliorated oleic acid-induced hepatic steatosis by inhibiting lipid accumulation and oxidative stress .