产品说明书
FAQ
COA
已发表文献
性能参数
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分子别名（Synonyms） |
MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1 |
|
表达区间及表达系统（Source） |
Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQV] |
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分子量大小（Molecular Weight） |
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-56 kDa based on SDS-PAGE result. |
|
内毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性（Activity） |
ELISA Data: Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Monomer, His Tag with the EC50 of 31.3ng/ml determined by ELISA. |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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重构方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
1.The product should be stored at -25~-15℃ for 1 year from date of receipt.
2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
