Integrin Beta 3 Recombinant Rabbit mAb [KD验证]
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The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
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推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000
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本产品仅用作科学研究!


Flow cytometric analysis of Integrin beta 3 expression in C2C12 cells using Integrin beta 3 antibody . Green, isotype control; red, Integrin beta 3.

Immunocytochemical staining of C2C12 cells with Integrin beta 3 antibody . Nuclei were stained blue with DAPI; Integrin beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Integrin β3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using integrin beta 3 antibody . Integrin beta 3 expression in wild-type (WT) and integrin beta 3 (ITGB3) shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with integrin beta 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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