The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using RAD23A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD23A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using RAD23A antibody . RAD23A expression in wild type (WT) and RAD23A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD23A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of RAD23A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAD23A antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of Hela cells using RAD23A antibody , Top panel: wild-type (WT); Bottom panal: RAD23A shRNA knockdown (KD). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of RAD23A expression in HepG2 cells using RAD23A antibody . Green, isotype control; red, RAD23A.

Immunocytochemical staining of HepG2 cells with RAD23A antibody . Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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