This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is transcriptional regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Western blotting analysis using MSI2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MSI2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using MSI2 antibody . MSI2 expression in wild type (WT) and MSI2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MSI2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of MSI2 expression in HepG2 cells using MSI2 antibody . Green, isotype control; red, MSI2.

Immunocytochemical staining of HepG2 cells with MSI2 antibody . Nuclei were stained blue with DAPI; MSI2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Validation of MSI2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MSI2 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using MSI2 antibody , Top panel: wild-type (WT); Bottom panal: MSI2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; MSI2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Store at -20℃ for one year.