This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

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Flow cytometric analysis of Phospho-Src (Y529) expression in C2C12 cells using Phospho-Src (Y529) antibody . Green, isotype control; red, Phospho-Src (Y529).

Immunocytochemical staining of HepG2 cells with Phospho-Src (Y529) antibody . Nuclei were stained blue with DAPI; Phospho-Src (Y529) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-Src (Y529) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Src (Y529) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-Src (Y529) antibody . Phospho-Src (Y529) expression in wild type (WT) and Phospho-Src (Y529) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-Src (Y529) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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